Experiment summary for ENCSR580TGX
doi:10.17989/ENCSR580TGX
Summary
- Status
- released
- Assay
- CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
- Target
- POLR2G
- Biosample summary
- Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens POLR2G
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on K562 cells treated with a CRISPR gRNA against POLR2G. (POLR2G-BGKcLV06)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.9 [1-1]9 [2-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR449DXG
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:POLR2G-BGKcLV06
- Date submitted
- November 20, 2017
- Date released
- December 6, 2017
- Gene Silencing Series
- ENCSR044VMR
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens POLR2G | ENCBS518EXZ | ENCGM865JVO | ENCLB620XUO |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens POLR2G | ENCBS767NRE | ENCGM865JVO | ENCLB428YIP |