Experiment summary for ENCSR572AWO
doi:10.17989/ENCSR572AWO
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- pat-9
- Biosample summary
- Caenorhabditis elegans strain RW12085 (pat-9(mNG^SEC^3xFlag::pat-9]) X) whole organism late embryo (3 hours) using egg bleaching genetically modified (insertion) using CRISPR targeting C. elegans pat-9
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing pat-9-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:PAT-9_RW12085_LE
- Date submitted
- February 26, 2018
- Date released
- March 1, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain RW12085 (pat-9(mNG^SEC^3xFlag::pat-9]) X) mixed sex late embryo (3 hours) using egg bleaching whole organism genetically modified (insertion) using CRISPR targeting C. elegans pat-9 | ENCBS772XGD | ENCGM655RLQ | ENCAB728YTO | ENCLB794HLC |
| 2 | 1 | Caenorhabditis elegans strain RW12085 (pat-9(mNG^SEC^3xFlag::pat-9]) X) mixed sex late embryo (3 hours) using egg bleaching whole organism genetically modified (insertion) using CRISPR targeting C. elegans pat-9 | ENCBS305MEK | ENCGM655RLQ | ENCAB728YTO | ENCLB151WWO |