Experiment summary for ENCSR538DNM

doi:10.17989/ENCSR538DNM

Summary

Status
released
Assay
RIP-seq
Target
U2af50
Biosample summary
Drosophila melanogaster strain Oregon-R S2R+ stably expressing U2af50 treated with 0.35 mM copper(II) sulfate for 24 hours
Biosample Type
cell line
Replication type
isogenic
Description
RIP-seq targeting U2af50 in Drosophila S2R+ cells.
Treatments
0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
Nucleic acid type
RNA
Size range
>200
Fragmentation methods
chemical (generic)
Size selection method
AMPure XP bead purification
Strand specificity
unstranded
Controls

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
References
Aliases
modencode:5429, brenton-graveley:S2R+-CG9998-HA_RIP
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal HA-tagged U2af50 under MtnA promoter, C-terminal FLAG-tagged U2af50 under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS430DSPENCGM912XMSENCAB639FUHENCLB722ZAH
21Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal HA-tagged U2af50 under MtnA promoter, C-terminal FLAG-tagged U2af50 under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS289LANENCGM912XMSENCAB639FUHENCLB738SZL