Experiment summary for ENCSR533CXT

doi:10.17989/ENCSR533CXT

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
BEAF-32
Biosample summary
Drosophila melanogaster strain BEAF-32-GFP whole organism embryo (0-14 hours) genetically modified (insertion) using site-specific recombination targeting dmelanogaster BEAF-32
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing BEAF-32-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:BEAF-32-GFP
Date submitted
September 11, 2017
Date released
November 22, 2017

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain BEAF-32-GFP mixed sex embryo (0-14 hours) whole organism genetically modified (insertion) using site-specific recombination targeting dmelanogaster BEAF-32ENCBS017ZBXENCGM153LRMENCAB728YTOENCLB818ZAJ
11strain BEAF-32-GFP mixed sex embryo (0-14 hours) whole organism genetically modified (insertion) using site-specific recombination targeting dmelanogaster BEAF-32ENCBS300HXEENCGM153LRMENCAB728YTOENCLB245ZMY
31strain BEAF-32-GFP mixed sex embryo (0-14 hours) whole organism genetically modified (insertion) using site-specific recombination targeting dmelanogaster BEAF-32ENCBS972JPOENCGM153LRMENCAB728YTOENCLB160ITG