Experiment summary for ENCSR525HSH

doi:10.17989/ENCSR525HSH

Summary

Status
released
Assay
polyA plus RNA-seq
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone for 25 minutes
Biosample Type
cell line
Replication type
isogenic
Description
mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 25 mins.
Treatments
100 nM dexamethasone (CHEBI:41879) for 25 minutes
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
>200
Extraction method
RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
Fragmentation methods
chemical (RNase III)
Size selection method
dual SPRI
Strand specificity
Strand-specific (reverse)

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
Aliases
tim-reddy:t25m_rna_seq
Date submitted
October 13, 2017
Date released
October 19, 2017

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21A549 cell line treated with 100 nM dexamethasone for 25 minutesENCBS126WELENCLB749UTI
31A549 cell line treated with 100 nM dexamethasone for 25 minutesENCBS810LXWENCLB879JTV
11A549 cell line treated with 100 nM dexamethasone for 25 minutesENCBS245ESTENCLB283EPI