Experiment summary for ENCSR518FUJ

doi:10.17989/ENCSR518FUJ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
pdm3
Biosample summary
Drosophila melanogaster strain pdm3-GFP whole organism prepupa at white prepupa stage genetically modified (insertion) using site-specific recombination targeting D. melanogaster pdm3
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing pdm3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:pdm3-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF672XXJ, ENCFF182VXQ were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain pdm3-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pdm3ENCBS253NLUENCGM052CJIENCAB728YTOENCLB844AWF
31Drosophila melanogaster strain pdm3-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pdm3ENCBS027YWKENCGM052CJIENCAB728YTOENCLB518FUG
21Drosophila melanogaster strain pdm3-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pdm3ENCBS638OJTENCGM052CJIENCAB728YTOENCLB468ZEM