Experiment summary for ENCSR514BBN

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP628 (wgIs628(ccch-3::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0161_4E04);unc-119(+));unc-119 (tm4063)) whole organism young adult (70 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing ccch-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CCCH-3_OP628_yAd
Date submitted
May 31, 2018
Date released
June 12, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP628 (wgIs628(ccch-3::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0161_4E04);unc-119(+));unc-119 (tm4063)) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-ccch-3ENCBS555JONENCGM953PPZENCAB728YTOENCLB814LBC
21strain OP628 (wgIs628(ccch-3::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0161_4E04);unc-119(+));unc-119 (tm4063)) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-ccch-3ENCBS286UDVENCGM953PPZENCAB728YTOENCLB966UOK