Experiment summary for ENCSR504QOC

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Mus musculus C57BL/6 bone marrow-derived macrophage nuclear fraction
Biosample Type
primary cell
Replication type
isogenic
Description
Ribo depletion RNA-seq on nucleoplasmic fraction RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for 30 minute.
Nucleic acid type
RNA
Depleted in
rRNA
Library starting quantity
1µg [1-1]500ng [2-1]
Size range
250-400
Extraction method
see document
Fragmentation method
see document
Size selection method
SPRI beads
Strand specificity
Non-strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
Aliases
alexander-hoffmann:Nuc.Naive.030.exp
Date submitted
January 20, 2017
Date released
November 20, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
11strain C57BL/6J bone marrow-derived macrophage primary cell nuclear fractionENCBS518CLYENCLB589DGP
21strain C57BL/6J bone marrow-derived macrophage primary cell nuclear fractionENCBS155NRAENCLB598WPV