Experiment summary for ENCSR500LPZ

doi:10.17989/ENCSR500LPZ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
K12H6.12
Biosample summary
Caenorhabditis elegans strain OP214 (unc-119(ed3) III; wgIs214) whole organism young adult (72 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans K12H6.12
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing K12H6.12-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:K12H6.12_OP214_yAd
Date submitted
February 26, 2018
Date released
March 1, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Caenorhabditis elegans strain OP214 (unc-119(ed3) III; wgIs214) mixed sex young adult (72 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans K12H6.12ENCBS753WZFENCGM237GLKENCAB728YTOENCLB397NBA
21Caenorhabditis elegans strain OP214 (unc-119(ed3) III; wgIs214) mixed sex young adult (72 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans K12H6.12ENCBS015NCUENCGM237GLKENCAB728YTOENCLB666GMC