Experiment summary for ENCSR497DHK

doi:10.17989/ENCSR497DHK

Summary

Status
released
Assay
RIP-seq
Target
msi
Biosample summary
Drosophila melanogaster strain Oregon-R S2R+ stably expressing msi treated with 0.35 mM copper(II) sulfate for 24 hours
Biosample Type
cell line
Replication type
isogenic
Description
RIP-seq targeting msi in Drosophila S2R+ cells.
Treatments
0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
Nucleic acid type
RNA
Size range
>200
Fragmentation methods
chemical (generic)
Size selection method
AMPure XP bead purification
Strand specificity
unstranded
Controls

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
References
Aliases
brenton-graveley:S2R+-CG5099-HA_RIP, modencode:5417
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged msi under MtnA promoter, C-terminal HA-tagged msi under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS795QWYENCGM665SMPENCAB639FUHENCLB214WMJ
11Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged msi under MtnA promoter, C-terminal HA-tagged msi under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS934YGZENCGM665SMPENCAB639FUHENCLB059EPE