Experiment summary for ENCSR490DYI

doi:10.17989/ENCSR490DYI

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
HNRNPLL
Biosample summary
Homo sapiens HepG2 expressing RNAi targeting H. sapiens HNRNPLL
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on HepG2 cells treated with an shRNA knockdown against HNRNPLL. (HNRNPLL_BGHLV35)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
Aliases
brenton-graveley:HNRNPLL_BGHLV35
Date submitted
September 25, 2016
Date released
September 29, 2016
Gene Silencing Series
ENCSR370RIN 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens HNRNPLLENCBS471COVENCGM524JDTENCLB303PMZ
21Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens HNRNPLLENCBS519IOBENCGM524JDTENCLB512WWM