Experiment summary for ENCSR485RQE
doi:10.17989/ENCSR485RQE
Summary
- Status
- released
- Assay
- siRNA knockdown followed by RNA-seq (siRNA RNA-seq)
- Target
- qkr58E-3
- Biosample summary
- Drosophila melanogaster strain Oregon-R S2 expressing RNAi targeting D. melanogaster qkr58E-3
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNAi knockdown against qkr58E-3 in S2-DRSC cell line followed by total RNA-seq (Oligo-dT selected, double stranded cDNA).
- Nucleic acid type
- RNA
- Size range
- >200
- Extraction method
- Trizol
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR358OMW
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modENCODE Project
- Lab
- Brenton Graveley, UConn
- Award
- U01HG004271 (Susan Celniker, LBNL)
- Project
- modENCODE
- External resources
- References
- Aliases
- brenton-graveley:S2-DRSC-CG3584-RNAi
- Date submitted
- March 31, 2014
- Date released
- March 31, 2014
- Gene Silencing Series
- ENCSR744EEZ
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain Oregon-R S2 cell line expressing RNAi targeting D. melanogaster qkr58E-3 | ENCBS833FAO | ENCGM889XLY | ENCLB069MMS |
| 1 | 1 | Drosophila melanogaster strain Oregon-R S2 cell line expressing RNAi targeting D. melanogaster qkr58E-3 | ENCBS991CJS | ENCGM889XLY | ENCLB608HEK |