Experiment summary for ENCSR482TZY

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone for 5 minutes
Biosample Type
cell line
Replication type
isogenic
Description
mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 5 mins.
Treatments
100 nM dexamethasone (CHEBI:41879) for 5 minute
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
>200
Extraction method
RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
Fragmentation method
chemical (RNase III)
Size selection method
dual SPRI
Strand specificity
Strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
Aliases
tim-reddy:t05m_rna_seq
Date submitted
October 13, 2017
Date released
October 19, 2017

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
31A549 cell line treated with 100 nM dexamethasone for 5 minutesENCBS353WHLENCLB229NCK
11A549 cell line treated with 100 nM dexamethasone for 5 minutesENCBS336AKRENCLB030TKL