Experiment summary for ENCSR477NIH
doi:10.17989/ENCSR477NIH
Summary
- Status
- released
- Assay
- small RNA-seq
- Biosample summary
- Drosophila melanogaster strain Oregon-R S2R+ nuclear fraction
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RNA-seq on rRNA-depleted total RNA in Drosophila cell line S2R+ (nuclear fraction), < 200 nucleotides in size that was CIP and TAP-treated prior to cloning.
- Treatments
- Antarctic Phosphatase for 30 minutes, Tobacco Acid Pyrophosphatase for 1 hour
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Size range
- <200
- Extraction method
- Trizol
- Strand specificity
- Strand-specific
- Platform
- Illumina Genome Analyzer IIx
Attribution
modENCODE Project
- Lab
- Thomas Gingeras, CSHL
- Award
- U01HG004271 (Susan Celniker, LBNL)
- Project
- modENCODE
- Aliases
- susan-celniker:E-BS599N-BS600N_CIPTAP, modencode:4217
- Date submitted
- March 31, 2014
- Date released
- March 31, 2014
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain Oregon-R S2R+ cell line nuclear fraction | ENCBS258AUT | ENCLB662HZC |
| 1 | 1 | Drosophila melanogaster strain Oregon-R S2R+ cell line nuclear fraction | ENCBS050FWY | ENCLB400BWL |