Experiment summary for ENCSR476OEL
doi:10.17989/ENCSR476OEL
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl neural tube tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse neural tube
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_NT
- Date submitted
- April 29, 2015
- Date released
- June 23, 2015
- Reference Epigenome
- ENCSR874LCF
- Organism Development Series
- ENCSR225OBZ
- Submitter comment
- Fastq files ENCFF814DRF, ENCFF428QDJ, ENCFF683ZED, and ENCFF861NXZ contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF814DRF: (20, 19306946), (37, 6863311); ENCFF428QDJ: (108, 206430827), (130, 218150863), (133, 216909793); ENCFF683ZED: (108, 206453529), (130, 207817747), (133, 219370916); ENCFF861NXZ (108, 216585401), (130, 218896372).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) neural tube tissue | ENCBS944KEZ | ENCLB457OTO |
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) neural tube tissue | ENCBS944KEZ | ENCLB804GKG |
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) neural tube tissue | ENCBS206NRE | ENCLB704NKX |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) neural tube tissue | ENCBS206NRE | ENCLB311FFB |