Experiment summary for ENCSR466TGH

doi:10.17989/ENCSR466TGH

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Target
DGCR8
Biosample summary
Homo sapiens K562 genetically modified (deletion) using CRISPR targeting H. sapiens DGCR8
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with a CRISPR gRNA against DGCR8 on day 22. (DGCR8-D22_BGKcLV02)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.9 [1-1]10 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:DGCR8-D22_BGKcLV02
Date submitted
March 15, 2016
Date released
March 23, 2016
Treatment Time Series
ENCSR111DOM 
Gene Silencing Series
ENCSR872RDF 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens DGCR8ENCBS626TPHENCGM467BBSENCLB385YIF
21Homo sapiens K562 cell line genetically modified (deletion) using CRISPR targeting H. sapiens DGCR8ENCBS732IZQENCGM467BBSENCLB280VKK