Experiment summary for ENCSR458VTP
doi:10.17989/ENCSR458VTP
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- NR3C1
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 20 minutes
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- GR ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 20 mins.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 20 minutes
- Nucleic acid type
- DNA
- Library starting quantity
- 7ng
- Size range
- >200
- Fragmentation methods
- sonication (Bioruptor Twin)
- Size selection method
- dual SPRI
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:GR.t20m
- Date submitted
- October 13, 2017
- Date released
- October 19, 2017
- Annotation (gkmsvm-model)
- Annotation (BPNet-model)
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
---|---|---|---|---|---|
2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 20 minutes | ENCBS686ZWW | ENCAB725CEF | ENCLB656TLU |
1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 20 minutes | ENCBS246FMQ | ENCAB725CEF | ENCLB969QJX |
3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 20 minutes | ENCBS145FXO | ENCAB725CEF | ENCLB625AUV |