Experiment summary for ENCSR450GPA

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
B0035.1
Biosample summary
Caenorhabditis elegans strain xtl1186 whole organism hermaphrodite young adult 50 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing B0035.1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:B0035.1-xtl1186-yAd
Date submitted
July 29, 2015
Date released
May 24, 2016
Submitter comment
ENCFF862KYX, ENCFF529RNI, ENCFF732VLZ, ENCFF975ECK were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain xtl1186 hermaphrodite young adult 50 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting B0035.1ENCBS092QNHENCGM540GYNENCAB728YTOENCLB073PWK
11strain xtl1186 hermaphrodite young adult 50 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting B0035.1ENCBS453WJSENCGM540GYNENCAB728YTOENCLB484PUH