Experiment summary for ENCSR438MDN

doi:10.17989/ENCSR438MDN

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Control type
non-specific target
Biosample summary
Homo sapiens K562 expressing RNAi
Biosample Type
cell line
Replication type
isogenic
Description
Control shRNA against no target in K562 cells followed by RNA-seq. (NT_BGKLV21-B)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.8 [1-1]10 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NT_BGKLV21-B
Date submitted
March 8, 2016
Date released
March 16, 2016
Gene Silencing Series
ENCSR409NTU 
, ENCSR463EDG 
, ENCSR406VZO 
, ENCSR596JUF 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAiENCBS800RVXENCGM266AWPENCLB101NSC
21Homo sapiens K562 cell line expressing RNAiENCBS530MLHENCGM266AWPENCLB114RXE