1. Experiments
  2. ChIP-seq
  3. Caenorhabditis elegans
  4. whole organism

Experiment summary for ENCSR435RXY

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
fkh-8
Biosample summary
Caenorhabditis elegans strain OP652 (unc-119(tm4063) III; wgIs652) whole organism L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing FKH-8-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:FKH-8-OP652-L1
Date submitted
October 4, 2016
Date released
October 10, 2016
Submitter comment
ENCFF069SMR, ENCFF977JKQ, ENCFF149QAJ, ENCFF701RXT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain OP652 (unc-119(tm4063) III; wgIs652) mixed sex L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting fkh-8ENCBS756SLFENCGM646PUKENCAB728YTOENCLB592EPN
11strain OP652 (unc-119(tm4063) III; wgIs652) mixed sex L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting fkh-8ENCBS072XKEENCGM646PUKENCAB728YTOENCLB923OSF