Experiment summary for ENCSR435RXY
Summary
- Status
- released
- Assay
- ChIP-seq
- Target
- eGFP-fkh-8
- Biosample summary
- Caenorhabditis elegans strain OP652 (unc-119(tm4063) III; wgIs652) whole organism L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing FKH-8-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation method
- sonication (generic microtip)
- Size selection method
- SPRI
- Strand specificity
- Non-strand-specific
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- Aliases
- kevin-white:FKH-8-OP652-L1
- Date submitted
- October 4, 2016
- Date released
- October 10, 2016
- Submitter comment
- ENCFF069SMR, ENCFF977JKQ, ENCFF149QAJ, ENCFF701RXT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
| Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | strain OP652 (unc-119(tm4063) III; wgIs652) mixed sex L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-fkh-8 | ENCBS756SLF | ENCGM646PUK | ENCAB728YTO | ENCLB592EPN |
| 1 | 1 | strain OP652 (unc-119(tm4063) III; wgIs652) mixed sex L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-fkh-8 | ENCBS072XKE | ENCGM646PUK | ENCAB728YTO | ENCLB923OSF |