Experiment summary for ENCSR434ALY

doi:10.17989/ENCSR434ALY

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
T23F11.4
Biosample summary
Caenorhabditis elegans strain OP709 (unc-119(tm4063); wgIs709 [T23F11.4::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism late embryo (4 hours) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans T23F11.4
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing T23F11.4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:T23F11.4_OP709_LE
Date submitted
February 26, 2018
Date released
March 1, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP709 (unc-119(tm4063); wgIs709 [T23F11.4::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex late embryo (4 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans T23F11.4ENCBS212WICENCGM113QBHENCAB728YTOENCLB707QRL
21Caenorhabditis elegans strain OP709 (unc-119(tm4063); wgIs709 [T23F11.4::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex late embryo (4 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans T23F11.4ENCBS764SOUENCGM113QBHENCAB728YTOENCLB497ZEB