Experiment summary for ENCSR432MSI

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP202 (unc-119(ed3) III; wgIs202) whole organism late embryo 4.75 hours post synchronization using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing CEH-9-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CEH-9-OP202-LE
Date submitted
October 4, 2016
Date released
October 10, 2016
Submitter comment
ENCFF775GLV, ENCFF146EQQ, ENCFF194RPX, ENCFF759YDE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP202 (unc-119(ed3) III; wgIs202) mixed sex late embryo 4.75 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-9ENCBS373XZJENCGM708ABHENCAB728YTOENCLB416MRA
21strain OP202 (unc-119(ed3) III; wgIs202) mixed sex late embryo 4.75 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-9ENCBS271XQLENCGM708ABHENCAB728YTOENCLB412NZS