Experiment summary for ENCSR428DFL
doi:10.17989/ENCSR428DFL
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K4me2
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 12 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- H3K4me2 ChIP-seq on A549 cell line treated with 100 nM dexamethasone for 12 hours.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 12 hours
- Nucleic acid type
- DNA
- Library starting quantity
- 7ng
- Size range
- >200
- Fragmentation methods
- sonication (Bioruptor Twin)
- Size selection method
- dual SPRI
- Platform
- Illumina HiSeq 2000, Illumina HiSeq 2500
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:H3K4me2.t12
- Date submitted
- May 30, 2016
- Date released
- July 21, 2016
- Treatment Time Series
- ENCSR868FCL
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS616EUG | ENCAB413RSR | ENCLB610JZP |
| 2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS154OIS | ENCAB413RSR | ENCLB557VLO |
| 3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 12 hours | ENCBS327IUO | ENCAB413RSR | ENCLB547RLX |