Experiment summary for ENCSR422FIJ
doi:10.17989/ENCSR422FIJ
Summary
- Status
- released
- Assay
- RIP-seq
- Target
- Srp54
- Biosample summary
- Drosophila melanogaster strain Oregon-R S2R+ stably expressing Srp54 treated with 0.35 mM copper(II) sulfate for 24 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- RIP-seq targeting Srp54 in Drosophila S2R+ cells.
- Treatments
- 0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
- Nucleic acid type
- RNA
- Size range
- >200
- Fragmentation methods
- chemical (generic)
- Size selection method
- AMPure XP bead purification
- Strand specificity
- unstranded
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modENCODE Project
- Lab
- Brenton Graveley, UConn
- Award
- U01HG004271 (Susan Celniker, LBNL)
- Project
- modENCODE
- External resources
- References
- Aliases
- brenton-graveley:S2R+-CG4602-HA_RIP, modencode:5415
- Date submitted
- March 31, 2014
- Date released
- March 31, 2014
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged Srp54 under MtnA promoter, C-terminal HA-tagged Srp54 under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hours | ENCBS475GAU | ENCGM654ZFR | ENCAB639FUH | ENCLB758VZL |
| 1 | 1 | Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged Srp54 under MtnA promoter, C-terminal HA-tagged Srp54 under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hours | ENCBS731WMM | ENCGM654ZFR | ENCAB639FUH | ENCLB410HFR |