Experiment summary for ENCSR420QTO

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Mus musculus C57BL/6 hindbrain embryo (12.5 days)
Biosample Type
tissue
Replication type
isogenic
Description
PolyA RNA-Seq from oligo-dT primed Total RNA on embryonic e12.5 day mouse hindbrain
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.6 [1-1]9.9 [2-1]
Library starting quantity
10.0ng
Size range
>200
Lysis method
Ambion mirVana
Extraction method
Ambion mirVana
Fragmentation method
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Spike-ins datasets
ENCSR884LPM

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Date submitted
April 19, 2016
Date released
May 3, 2016
Related datasets
ENCSR605BEG 
Reference epigenome for mouse hindbrain (embryonic 12.5 day).
Submitter comment
The lab submitted deeper sequencing; old processed data was archived on 11/02/2016/ and the raw data will be reprocessed.
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
11strain B6NCrl mixed sex embryo (12.5 days) hindbrain tissueENCBS406RIEENCLB408EFI
21strain B6NCrl mixed sex embryo (12.5 days) hindbrain tissueENCBS022IBYENCLB008IOE