Experiment summary for ENCSR410UOB

doi:10.17989/ENCSR410UOB

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Eip75B
Biosample summary
Drosophila melanogaster strain Eip75B-MiMIC-GFP whole organism prepupa genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75B
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Eip75B-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Eip75B-MIMIC-GFP
Date submitted
October 6, 2015
Date released
July 6, 2016
Submitter comment
ENCFF877FOL, ENCFF572YMK, ENCFF032WOR, ENCFF331NQT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain Eip75B-MiMIC-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75BENCBS465JCGENCGM886AMOENCAB728YTOENCLB266WHS
11Drosophila melanogaster strain Eip75B-MiMIC-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75BENCBS424OOOENCGM886AMOENCAB728YTOENCLB043NVY
21Drosophila melanogaster strain Eip75B-MiMIC-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75BENCBS166WBXENCGM886AMOENCAB728YTOENCLB978IEW