Experiment summary for ENCSR410UOB
doi:10.17989/ENCSR410UOB
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- Eip75B
- Biosample summary
- Drosophila melanogaster strain Eip75B-MiMIC-GFP whole organism prepupa genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75B
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing Eip75B-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:Eip75B-MIMIC-GFP
- Date submitted
- October 6, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF877FOL, ENCFF572YMK, ENCFF032WOR, ENCFF331NQT were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
3 | 1 | Drosophila melanogaster strain Eip75B-MiMIC-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75B | ENCBS465JCG | ENCGM886AMO | ENCAB728YTO | ENCLB266WHS |
1 | 1 | Drosophila melanogaster strain Eip75B-MiMIC-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75B | ENCBS424OOO | ENCGM886AMO | ENCAB728YTO | ENCLB043NVY |
2 | 1 | Drosophila melanogaster strain Eip75B-MiMIC-GFP mixed sex prepupa whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Eip75B | ENCBS166WBX | ENCGM886AMO | ENCAB728YTO | ENCLB978IEW |