Experiment summary for ENCSR397XGG

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP391 (unc-119(ed3) III; wgIs391(med-1::TY1 EGFP FLAG C; unc-119(+))) whole organism hermaphrodite midembryo genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing MED-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
eGEL
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:MED-1-OP391-ME
Date submitted
July 30, 2015
Date released
November 22, 2017
Submitter comment
This experiment was completed during modENCODE and has lower read depth than the current modERN standard.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP391 (unc-119(ed3) III; wgIs391(med-1::TY1 EGFP FLAG C; unc-119(+))) hermaphrodite midembryo whole organism genetically modified (insertion) using bombardment targeting eGFP-med-1ENCBS609DZCENCGM933PCYENCAB435FBQENCLB285URE
21strain OP391 (unc-119(ed3) III; wgIs391(med-1::TY1 EGFP FLAG C; unc-119(+))) hermaphrodite midembryo whole organism genetically modified (insertion) using bombardment targeting eGFP-med-1ENCBS841UVRENCGM933PCYENCAB435FBQENCLB367QPF