Experiment summary for ENCSR393QBI

doi:10.17989/ENCSR393QBI

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
BtbVII
Biosample summary
Drosophila melanogaster strain BtbVII-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster BtbVII
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing BtbVII-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:BtbVII-GFP
Date submitted
December 24, 2016
Date released
January 11, 2017
Submitter comment
ENCFF044LEO, ENCFF245RWI, ENCFF589EBV, ENCFF322UWM were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain BtbVII-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster BtbVIIENCBS137PNEENCGM173ELVENCAB728YTOENCLB743UDX
11Drosophila melanogaster strain BtbVII-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster BtbVIIENCBS641HDEENCGM173ELVENCAB728YTOENCLB919INK
21Drosophila melanogaster strain BtbVII-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster BtbVIIENCBS478VVPENCGM173ELVENCAB728YTOENCLB481WFA