Experiment summary for ENCSR389SSP

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP756 (unc-119(tm4063); wgIs756 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism early embryo (2 hours) using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing CEH-13-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
valerie-reinke:CEH-13_OP756_EE
Date submitted
September 17, 2018
Date released
September 19, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP756 (unc-119(tm4063); wgIs756 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex early embryo (2 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-13ENCBS424SAGENCGM130FKKENCAB728YTOENCLB861SYO
21strain OP756 (unc-119(tm4063); wgIs756 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex early embryo (2 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-13ENCBS876WCLENCGM130FKKENCAB728YTOENCLB962WYS