Experiment summary for ENCSR385UPQ

doi:10.17989/ENCSR385UPQ

Supersedes ENCSR627NVU

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
RBM15
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens RBM15
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against RBM15. (RBM15_BGKLV32)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.7 [1-1]9.6 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:RBM15_BGKLV32
Date submitted
May 26, 2016
Date released
June 13, 2016
Gene Silencing Series
ENCSR676VUZ 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens RBM15ENCBS808FZKENCGM321DXWENCLB813JOE
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens RBM15ENCBS271BPHENCGM321DXWENCLB936SHB