Experiment summary for ENCSR384KCZ

doi:10.17989/ENCSR384KCZ

Summary

Status
released
Assay
DNase-seq
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 1 hour
Biosample Type
cell line
Replication type
isogenic
Description
DNase-seq on A549 cell line treated with 100 nM dexamethasone for 1 hours.
Treatments
100 nM dexamethasone (CHEBI:41879) for 1 hour
Nucleic acid type
DNA
Library starting quantity
236ng [1-1]262ng [3-1]
Size selection method
gel
Controls

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
External resources
Aliases
tim-reddy:t1_dnase_seq
Date submitted
March 2, 2016
Date released
May 5, 2016
Treatment Time Series
ENCSR482QFS 
Annotation (gkmsvm-model)
Submitter comment
DNase-seq libraries were sequenced with four barcodes per lane to generate 50 bp single-end reads, of which only the first 20 bp were genomic DNA due to MmeI digestion (see Song and Crawford 2010).
Tags
Search for all Experiment with internal tag RegulomeDB_1_0Search for all Experiment with internal tag RegulomeDB_2_2Search for all Experiment with internal tag RegulomeDB_2_0Search for all Experiment with internal tag RegulomeDB_2_1

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 1 hourENCBS960LFKENCLB804YIG
21Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 1 hourENCBS772RGZENCLB551XJI
41Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 1 hourENCBS691ANIENCLB471SDC
51Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 1 hourENCBS606CKRENCLB699SEB
31Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 1 hourENCBS887WBAENCLB045CXE