Experiment summary for ENCSR384BDV

doi:10.17989/ENCSR384BDV

Supersedes ENCSR895BTE

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
CPSF6
Biosample summary
Homo sapiens K562 expressing RNAi targeting H. sapiens CPSF6
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with an shRNA knockdown against CPSF6. (CPSF6_BGKLV36)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:CPSF6_BGKLV36
Date submitted
September 25, 2016
Date released
September 29, 2016
Gene Silencing Series
ENCSR232HNS 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAi targeting H. sapiens CPSF6ENCBS936QKMENCGM917PVSENCLB635WXU
21Homo sapiens K562 cell line expressing RNAi targeting H. sapiens CPSF6ENCBS967ZCFENCGM917PVSENCLB486NVV