Experiment summary for ENCSR374HBD
doi:10.17989/ENCSR374HBD
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- hnd-1
- Biosample summary
- Caenorhabditis elegans strain OP573 (unc-119(tm4063) III; wgIs573) whole organism early embryo 1.5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans hnd-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing HND-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:HND-1-OP573-EE
- Date submitted
- October 4, 2016
- Date released
- October 27, 2016
- Submitter comment
- ENCFF291QAZ, ENCFF311YNR, ENCFF832ZUJ, ENCFF462VDM were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OP573 (unc-119(tm4063) III; wgIs573) mixed sex early embryo 1.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hnd-1 | ENCBS206MPC | ENCGM856QXU | ENCAB728YTO | ENCLB332TGG |
| 1 | 1 | Caenorhabditis elegans strain OP573 (unc-119(tm4063) III; wgIs573) mixed sex early embryo 1.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hnd-1 | ENCBS158RMI | ENCGM856QXU | ENCAB728YTO | ENCLB804WFF |