Experiment summary for ENCSR366QZI
doi:10.17989/ENCSR366QZI
Summary
- Status
- archived
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Biosample summary
- Homo sapiens Purkinje cell male adult (20 years)
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- RNA-seq of a single Purkinje cell from a 20 year old human male
- Nucleic acid type
- RNA
- Library starting quantity
- 1cells
- Size range
- >200
- Lysis method
- 72 degrees for 3 minutes in the presence of Triton
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ, ENCSR133ALU
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Aliases
- barbara-wold:single-cell-13637-exp
- Date submitted
- May 13, 2014
- Date released
- July 7, 2014
- Submitter comment
- Estimates of technical measurement error from low input mass RNA-seq libraries.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Homo sapiens male adult (20 years) Purkinje primary cell | ENCBS982VXC | ENCLB350NUB |