Experiment summary for ENCSR366QZI

doi:10.17989/ENCSR366QZI

Summary

Status
archived
Assay
single-cell RNA sequencing assay (scRNA-seq)
Biosample summary
Homo sapiens Purkinje cell male adult (20 years)
Biosample Type
primary cell
Replication type
unreplicated
Description
RNA-seq of a single Purkinje cell from a 20 year old human male
Nucleic acid type
RNA
Library starting quantity
1cells
Size range
>200
Lysis method
72 degrees for 3 minutes in the presence of Triton
Fragmentation methods
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Strand specificity
unstranded
Spike-ins datasets
ENCSR013YHQ, ENCSR133ALU
Controls

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Aliases
barbara-wold:single-cell-13637-exp
Date submitted
May 13, 2014
Date released
July 7, 2014
Submitter comment
Estimates of technical measurement error from low input mass RNA-seq libraries.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens male adult (20 years) Purkinje primary cellENCBS982VXCENCLB350NUB