Experiment summary for ENCSR365IMJ

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Drosophila melanogaster VK00033 whole organism embryo (2-10 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing dmrt11E-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:dmrt11E-GFP
Date released
December 21, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
31mixed sex embryo (2-10 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-dmrt11EENCBS502DCSENCGM994BMBENCAB728YTOENCLB391TFU
11mixed sex embryo (2-10 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-dmrt11EENCBS828NBZENCGM994BMBENCAB728YTOENCLB964NBK
21mixed sex embryo (2-10 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-dmrt11EENCBS900ANDENCGM994BMBENCAB728YTOENCLB412DTO