Experiment summary for ENCSR360TFL

doi:10.17989/ENCSR360TFL

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
CG31388
Biosample summary
Drosophila melanogaster strain CG31388-GFP whole organism embryo (0-16 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG31388
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CG31388-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:CG31388-GFP
Date submitted
April 9, 2016
Date released
July 6, 2016
Submitter comment
ENCFF648EWG, ENCFF991DUD were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41Drosophila melanogaster strain CG31388-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG31388ENCBS275UMTENCGM588NRWENCAB728YTOENCLB397NOJ
21Drosophila melanogaster strain CG31388-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG31388ENCBS239QKMENCGM588NRWENCAB728YTOENCLB773PKS