Experiment summary for ENCSR341VFG

doi:10.17989/ENCSR341VFG

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Homo sapiens activated T-helper 2 cell male adult (35 years) treated with 100 ng/mL Interleukin-4, anti-CD3 and anti-CD28 coated beads, 5 μg/mL Interferon-gamma antibody
Biosample Type
primary cell
Replication type
isogenic
Description
CD4 N (RA+ MACS sort)Th2 (aIFNg 5ug/ml+IL-4 100ng/ml)(day 13)+beads (1:3) day 1
Treatments
100 ng/mL Interleukin-4 (UniProtKB:P05112) , anti-CD3 and anti-CD28 coated beads , 5 μg/mL Interferon-gamma antibody
Nucleic acid type
RNA
RNA integrity number
9
Library starting quantity
300ng
Average fragment size
279 [1-1]278 [2-1]
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR597UQL

Attribution

ENCODE4 project
Lab
Barbara Wold, Caltech
Award
UM1HG009443 (Barbara Wold, Caltech)
Project
ENCODE
Date released
July 14, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens male adult (35 years) activated T-helper 2 primary cell treated with 100 ng/mL Interleukin-4, anti-CD3 and anti-CD28 coated beads, 5 μg/mL Interferon-gamma antibodyENCBS309SJIENCLB013PVL
21Homo sapiens male adult (35 years) activated T-helper 2 primary cell treated with 100 ng/mL Interleukin-4, anti-CD3 and anti-CD28 coated beads, 5 μg/mL Interferon-gamma antibodyENCBS696MEHENCLB499IUU