Experiment summary for ENCSR341VFG
doi:10.17989/ENCSR341VFG
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Homo sapiens activated T-helper 2 cell male adult (35 years) treated with 5 μg/mL Interferon-gamma antibody for 36 hours, anti-CD3 and anti-CD28 coated beads for 24 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 100 ng/mL Interleukin-4 for 36 hours
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- CD4 N (RA+ MACS sort)Th2 (aIFNg 5ug/ml+IL-4 100ng/ml)(day 13)+beads (1:3) day 1
- Treatments
- 5 μg/mL Interferon-gamma antibody for 36 hours, anti-CD3 and anti-CD28 coated beads for 24 hours, 10 ng/mL Interleukin-2 (UniProtKB:P60568) for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 100 ng/mL Interleukin-4 (UniProtKB:P05112) for 36 hours
- Nucleic acid type
- RNA
- RNA integrity number
- 9
- Library starting quantity
- 300ng
- Average fragment size
- 279 [1-1]278 [2-1]
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR597UQL
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE4 project
- Lab
- Barbara Wold, Caltech
- Award
- UM1HG009443 (Barbara Wold, Caltech)
- Project
- ENCODE
- External resources
- Date released
- July 14, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (35 years) activated T-helper 2 primary cell treated with 5 μg/mL Interferon-gamma antibody for 36 hours, anti-CD3 and anti-CD28 coated beads for 24 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 100 ng/mL Interleukin-4 for 36 hours | ENCBS309SJI | ENCLB013PVL |
| 2 | 1 | Homo sapiens male adult (35 years) activated T-helper 2 primary cell treated with 5 μg/mL Interferon-gamma antibody for 36 hours, anti-CD3 and anti-CD28 coated beads for 24 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 100 ng/mL Interleukin-4 for 36 hours | ENCBS696MEH | ENCLB499IUU |