1. Experiments
  2. CRISPR genome editing followed by RNA-seq
  3. Homo sapiens
  4. K562

Experiment summary for ENCSR341TTW

doi:10.17989/ENCSR341TTW

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Control type
non-specific target
Biosample summary
Homo sapiens K562 genetically modified (deletion) using CRISPR
Biosample Type
cell line
Replication type
isogenic
Description
Control CRISPR gRNA against no target in K562 cells followed by RNA-seq. (NT-BGKcLV06)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.5 [2-1]9.8 [1-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR449DXG

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NT-BGKcLV06
Date submitted
November 20, 2017
Date released
December 6, 2017
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens K562 cell line genetically modified (deletion) using CRISPRENCBS044NCUENCGM474NKSENCLB459AVL
11Homo sapiens K562 cell line genetically modified (deletion) using CRISPRENCBS280LUDENCGM474NKSENCLB559SJO

Files

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Replicates

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