Experiment summary for ENCSR340RMP

doi:10.17989/ENCSR340RMP

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Drosophila melanogaster strain Oregon-R S2
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on rRNA-depleted total RNA in Drosophila cell line S2-DRSC, < 200 nucleotides in size that was CIP and TAP-treated prior to cloning.
Treatments
Antarctic Phosphatase for 30 minutes, Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Extraction method
Trizol
Strand specificity
Strand-specific

Attribution

modENCODE Project
Lab
Thomas Gingeras, CSHL
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
Aliases
susan-celniker:E-BS597WC-BS598WC_CIPTAP, modencode:4219
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21Drosophila melanogaster strain Oregon-R S2 cell lineENCBS339BLCENCLB858FPG
11Drosophila melanogaster strain Oregon-R S2 cell lineENCBS227VZLENCLB324OSL