1. Experiments
  2. ChIP-seq
  3. Caenorhabditis elegans
  4. whole organism

Experiment summary for ENCSR338HCL

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
nhr-25
Biosample summary
Caenorhabditis elegans strain OP33 (unc-119(ed3) III; wgIs33) whole organism hermaphrodite L4 larva genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing NHR-25-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
eGEL
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:NHR-25-OP33-L4
Date submitted
July 30, 2015
Date released
November 22, 2017
Submitter comment
This experiment was completed during modENCODE and has lower read depth than the current modERN standard.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11strain OP33 (unc-119(ed3) III; wgIs33) hermaphrodite L4 larva whole organism genetically modified (insertion) using bombardment targeting nhr-25ENCBS781FUAENCGM756IDKENCAB435FBQENCLB046RRH
21strain OP33 (unc-119(ed3) III; wgIs33) hermaphrodite L4 larva whole organism genetically modified (insertion) using bombardment targeting nhr-25ENCBS499OQEENCGM756IDKENCAB435FBQENCLB853SWY