Experiment summary for ENCSR338HCL
Summary
- Status
- released
- Assay
- ChIP-seq
- Target
- eGFP-nhr-25
- Biosample summary
- Caenorhabditis elegans strain OP33 (unc-119(ed3) III; wgIs33) whole organism hermaphrodite L4 larva genetically modified using bombardment
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing NHR-25-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation method
- sonication (generic microtip)
- Size selection method
- eGEL
- Strand specificity
- Non-strand-specific
- Platform
- Illumina Genome Analyzer IIx
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- Aliases
- kevin-white:NHR-25-OP33-L4
- Date submitted
- July 30, 2015
- Date released
- November 22, 2017
- Submitter comment
- This experiment was completed during modENCODE and has lower read depth than the current modERN standard.
Isogenic replicates
| Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | strain OP33 (unc-119(ed3) III; wgIs33) hermaphrodite L4 larva whole organism genetically modified (insertion) using bombardment targeting eGFP-nhr-25 | ENCBS781FUA | ENCGM756IDK | ENCAB435FBQ | ENCLB046RRH |
| 2 | 1 | strain OP33 (unc-119(ed3) III; wgIs33) hermaphrodite L4 larva whole organism genetically modified (insertion) using bombardment targeting eGFP-nhr-25 | ENCBS499OQE | ENCGM756IDK | ENCAB435FBQ | ENCLB853SWY |