Experiment summary for ENCSR335NNR

doi:10.17989/ENCSR335NNR

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
nerfin-1
Biosample summary
Drosophila melanogaster strain nerfin-1 whole organism embryo (4-16 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster nerfin-1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing -eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:nerfin-1-GFP
Date submitted
April 12, 2019
Date released
May 23, 2019

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain nerfin-1 mixed sex embryo (4-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster nerfin-1ENCBS753SSIENCGM025MGUENCAB728YTOENCLB205XUP
11Drosophila melanogaster strain nerfin-1 mixed sex embryo (4-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster nerfin-1ENCBS527RLLENCGM025MGUENCAB728YTOENCLB322KES
21Drosophila melanogaster strain nerfin-1 mixed sex embryo (4-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster nerfin-1ENCBS055SRVENCGM025MGUENCAB728YTOENCLB212MIA