Experiment summary for ENCSR326PTW
doi:10.17989/ENCSR326PTW
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 4 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 4 hours.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 4 hours
- Nucleic acid type
- polyadenylated mRNA
- Library starting quantity
- 1µg
- Size range
- >200
- Extraction method
- RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
- Size selection method
- dual SPRI
- Strand specificity
- Strand-specific (forward)
- Platform
- Illumina HiSeq 2500, Illumina HiSeq 2000
- Controls
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:t4_rna_seq
- Date submitted
- March 3, 2016
- Date released
- May 5, 2016
- Treatment Time Series
- ENCSR897XFT
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 4 hours | ENCBS023FRY | ENCLB808GGW |
2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 4 hours | ENCBS167GTR | ENCLB517NAM |
3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 4 hours | ENCBS700HNY | ENCLB529EZQ |
4 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 4 hours | ENCBS070TPK | ENCLB617ROP |