Experiment summary for ENCSR324WIS
doi:10.17989/ENCSR324WIS
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Control type
- non-specific target
- Biosample summary
- Homo sapiens K562 expressing RNAi
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- Control shRNA against no target in K562 cells followed by RNA-seq. (NT-BGKLV36-B)
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 10 [2-1]9.4 [1-1]
- Size range
- >200
- Extraction method
- Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award
- U54HG007005 (Brenton Graveley, UConn)
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:NT-BGKLV36-B
- Date submitted
- November 20, 2017
- Date released
- December 6, 2017
- Gene Silencing Series
- ENCSR718WJL
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS131LFM | ENCGM266AWP | ENCLB995VIM |
| 1 | 1 | Homo sapiens K562 cell line expressing RNAi | ENCBS405CUM | ENCGM266AWP | ENCLB059RKL |