Experiment summary for ENCSR324NAF
doi:10.17989/ENCSR324NAF
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl liver tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse liver
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_LV
- Date submitted
- April 29, 2015
- Date released
- June 23, 2015
- Organism Development Series
- ENCSR369AYR
- Reference Epigenome
- ENCSR871WIE
- Submitter comment
- Fastq files ENCFF271ZHP, ENCFF047YWY, ENCFF162EBN, and ENCFF462BPF contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF271ZHP (109, 168448890), (117, 194230830), (133, 210410675), (139, 168448890); ENCFF047YWY (109, 184220095), (117, 203659469), (130, 169162566), (139, 184220095); ENCFF162EBN (109, 185664372), (117, 142554081), (133, 196120131), (139, 185664372); ENCFF462BPF (109, 205194864), (117, 143420382), (130, 194973406), (139, 205194864).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) liver tissue | ENCBS734ENZ | ENCLB220IVH |
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) liver tissue | ENCBS734ENZ | ENCLB298RHH |
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) liver tissue | ENCBS966PAM | ENCLB731OQN |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) liver tissue | ENCBS966PAM | ENCLB576UBZ |