1. Experiments
  2. shRNA knockdown followed by RNA-seq
  3. Drosophila melanogaster
  4. whole organism

Experiment summary for ENCSR315VLR

doi:10.17989/ENCSR315VLR

Alternate accession: ENCSR361OTE

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Target
twi
Biosample summary
Drosophila melanogaster strain twi_shRNA x MTD-Gal4 whole organism embryo 16-18 hours post synchronization at egg laying stage expressing RNAi targeting D. melanogaster twi
Biosample Type
whole organisms
Replication type
isogenic
Nucleic acid type
RNA
Depleted in
polyadenylated mRNA
Size range
350-600
Extraction method
Trizol
Fragmentation methods
shearing (Covaris generic)
Strand specificity
unstranded
Controls

Attribution

modERN project
Lab
Susan Celniker, LBNL
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
susan-celniker:twi_16-18
Date submitted
March 15, 2019
Date released
April 25, 2019
Submitter comment
Processed data is currently not available for these experiments, but will be added in the future.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Drosophila melanogaster strain twi_shRNA x MTD-Gal4 embryo 16-18 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster twiENCBS556AOTENCGM265FCTENCLB173MWF
21Drosophila melanogaster strain twi_shRNA x MTD-Gal4 embryo 16-18 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster twiENCBS380GMWENCGM265FCTENCLB422XUW

Files

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