Experiment summary for ENCSR298RJD

doi:10.17989/ENCSR298RJD

Summary

Status
released
Assay
siRNA knockdown followed by RNA-seq (siRNA RNA-seq)
Target
barc
Biosample summary
Drosophila melanogaster strain Oregon-R S2 expressing RNAi targeting D. melanogaster barc
Biosample Type
cell line
Replication type
isogenic
Description
RNAi knockdown against barc in S2-DRSC cell line followed by total RNA-seq (Oligo-dT selected, double stranded cDNA).
Nucleic acid type
RNA
Size range
>200
Extraction method
Trizol
Strand specificity
unstranded
Spike-ins datasets
ENCSR358OMW

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
External resources
References
Aliases
brenton-graveley:S2-DRSC-CG6049-RNAi
Date submitted
March 31, 2014
Date released
March 31, 2014
Gene Silencing Series
ENCSR004DEZ 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Drosophila melanogaster strain Oregon-R S2 cell line expressing RNAi targeting D. melanogaster barcENCBS142TUNENCGM858JNMENCLB138OSE
21Drosophila melanogaster strain Oregon-R S2 cell line expressing RNAi targeting D. melanogaster barcENCBS161TOOENCGM858JNMENCLB337NBL