Experiment summary for ENCSR295GRP

doi:10.17989/ENCSR295GRP

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
br
Biosample summary
Drosophila melanogaster strain br-re-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster br
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing br-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:br-RE-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF823RNL, ENCFF932HAT, ENCFF224JPO, ENCFF091QQO were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41Drosophila melanogaster strain br-re-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster brENCBS873QPKENCGM431MXXENCAB728YTOENCLB832HZA
31Drosophila melanogaster strain br-re-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster brENCBS610GTTENCGM431MXXENCAB728YTOENCLB350DOB
21Drosophila melanogaster strain br-re-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster brENCBS925VJOENCGM431MXXENCAB728YTOENCLB700PUG