Experiment summary for ENCSR294XUZ

doi:10.17989/ENCSR294XUZ

Summary

Status
released
Assay
DNase-seq
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 3 hours
Biosample Type
cell line
Replication type
isogenic
Description
DNase-seq on A549 cell line treated with 100 nM dexamethasone for 3 hours.
Treatments
100 nM dexamethasone (CHEBI:41879) for 3 hours
Nucleic acid type
DNA
Library starting quantity
204ng
Size selection method
gel
Controls

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
External resources
Aliases
tim-reddy:t3_dnase_seq
Date submitted
December 16, 2015
Date released
May 5, 2016
Treatment Time Series
ENCSR482QFS 
Annotation (gkmsvm-model)
Submitter comment
DNase-seq libraries were sequenced with four barcodes per lane to generate 50 bp single-end reads, of which only the first 20 bp were genomic DNA due to MmeI digestion (see Song and Crawford 2010).
Tags
Search for all Experiment with internal tag RegulomeDB_1_0Search for all Experiment with internal tag RegulomeDB_2_2Search for all Experiment with internal tag RegulomeDB_2_0Search for all Experiment with internal tag RegulomeDB_2_1

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hoursENCBS006TYDENCLB030QJR
51Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hoursENCBS497NSMENCLB130MQZ
11Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hoursENCBS721JKRENCLB997XQZ
31Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 3 hoursENCBS875AZAENCLB118QQR