Experiment summary for ENCSR294OOK

doi:10.17989/ENCSR294OOK

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Mus musculus strain C57BL/6J macrophage nuclear fraction
Biosample Type
primary cell
Replication type
isogenic
Description
Ribo depletion RNA-seq on nucleoplasmic fraction RNA which is part of BMDM cell pre-treated with 100 ng/ml Lipid A 12 hours, washed out for 12 hours, and stimulated with 100 ng/ml Lipid A for 120 minute.
Nucleic acid type
RNA
Depleted in
rRNA
Library starting quantity
1µg [1-1]500ng [2-1]
Size range
250-400
Fragmentation methods
see document
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
External resources
Aliases
alexander-hoffmann:Nuc.LPA.120.exp
Date submitted
January 20, 2017
Date released
November 20, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Mus musculus strain C57BL/6J macrophage primary cell nuclear fractionENCBS177LDSENCLB187VNP
21Mus musculus strain C57BL/6J macrophage primary cell nuclear fractionENCBS013YIUENCLB882OJA