Experiment summary for ENCSR292NVQ

doi:10.17989/ENCSR292NVQ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
hlh-12
Biosample summary
Caenorhabditis elegans strain OP538 (unc-119(tm4063) III; wgIs538) whole organism hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans hlh-12
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing HLH-12-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:HLH-12-OP538-yAd
Date submitted
July 29, 2015
Date released
May 10, 2016
Submitter comment
ENCFF367NVV, ENCFF217OHV, ENCFF157GFO, ENCFF484KOL were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP538 (unc-119(tm4063) III; wgIs538) hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans hlh-12ENCBS803EZPENCGM648OAVENCAB728YTOENCLB536VHL
11Caenorhabditis elegans strain OP538 (unc-119(tm4063) III; wgIs538) hermaphrodite young adult 64 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans hlh-12ENCBS246BHRENCGM648OAVENCAB728YTOENCLB892KXV